The HRP-conjugated Goat anti-Mouse IgG2a (CABS065) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in goats, is specifically designed to target IgG2a antibodies in mouse samples, enabling reliable detection and analysis in various experimental settings.With its high reactivity and specificity for IgG2a antibodies, this HRP-conjugated Goat Anti-Mouse antibody is ideal for use in Western blot applications, ELISA assays, and immunohistochemistry studies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
HRP-conjugated Goat anti-Mouse IgG2a
SKU:
CABS065
Size:
100μL, 200μL
Reactivity:
Mouse
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:5000
ELISA
1:2000 - 1:5000
Positive Sample:
Mouse IgG2a
Observed MW:
25kDa(Lightchain),55kDa(Heavychain)
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2769853
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 50% glycerol,pH7.4.
Western blot analysis of lysates from Mouse IgG using HRP Goat Anti-Mouse IgG2a (CABS065) at 1:2000 dilution. Lysates/proteins: 100ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
