The HRP-conjugated Mouse anti DDDDK-Tag mAb (CABE024) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in mice, exhibits high specificity and sensitivity for detecting the DDDDK tag in various experimental settings. With its high affinity for the DDDDK tag protein, this monoclonal antibody enables accurate and reliable detection of tagged proteins in Western blot, immunoprecipitation, and immunofluorescence assays. Its versatility and robust performance make it an essential reagent for studies involving protein expression, purification, and localization.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
HRP-conjugated Mouse anti DDDDK-Tag mAb
SKU:
CABE024
Size:
50μL, 100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0382-HRP
Conjugate:
HRP
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
DYKD DDDK
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:5000 - 1:25000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Purification Method
Affinity purification
RRID
AB_2769864
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293F cells transfected with IRF1-Flag protein , using HRP-conjugated Mouse anti DDDDK-Tag mAb (CABE024) at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from 293T cells transfected with GSK3B-Flag protein using HRP-conjugated Mouse anti DDDDK-Tag mAb (CABE024) at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
