The HRP-conjugated Mouse anti Myc-Tag mAb (CABE026) is a high-quality antibody developed for reliable detection and analysis of target proteins. Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
HRP-conjugated Mouse anti Myc-Tag mAb
SKU:
CABE026
Size:
100μL
Reactivity:
Species independent
Clone Number:
AMC0048
Conjugate:
HRP
Immunogen:
A synthetic peptide corresponding to Myc tag.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:5000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Myc, Myc tag, Myc-tag
Positive Sample:
293T transfected with NLK -Myc-Tag, 293T transfected with PTEN-Myc-Tag
Observed MW:
55 kDa/58 kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2769869
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NLK -Myc-Tag, 293F cells transfected with PTEN-Myc-Tag using HRP-conjugated Mouse anti Myc-Tag mAb (CABE026) at 1:5000 dilution incubated overnight at 4℃. Lysates/proteins: 20 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1 s.
