The HRP-conjugated Mouse anti-Rabbit IgG Light Chain (CABS061) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in mice, is highly specific for rabbit IgG light chain and is validated for use in a variety of applications, including Western blotting.CD300A, also known as an immune inhibitory receptor, plays a critical role in immune homeostasis by regulating inflammation and inhibiting allergic reactions. By targeting this protein with the HRP-conjugated Mouse Anti-Rabbit IgG Light Chain antibody, researchers can gain valuable insights into immune response modulation in various cell types, making it ideal for studies in immunology and cancer research.
This antibody is validated for use in WB, IP applications and has demonstrated reactivity against Rabbit samples.
Product Name:
HRP-conjugated Mouse anti-Rabbit IgG Light Chain
SKU:
CABS061
Size:
50μL, 100μL, 200μL
Reactivity:
Rabbit
Clone Number:
AMC0531
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WBIP
Recommended Dilution:
WB
,1:5000 - 1:10000
Positive Sample:
Rabbit control IgG
Observed MW:
25kDa(Lightchain)
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2864055
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (CABS061) at 1:10000 dilution. Lysates/proteins: 100 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg IKKα antibody (CAB2062' target='_blank' >A2062). Western blot was performed from the immunoprecipitate using IKKα antibody (CAB2062' target='_blank' >A2062) at a dilution of 1:1000. Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (CABS061) at 1:20000 dilution.
