The HRP-conjugated Mouse anti-Rabbit IgG Light Chain (CABS061) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
This antibody is validated for use in WB, IP applications and has demonstrated reactivity against Rabbit samples.
Product Name:
HRP-conjugated Mouse anti-Rabbit IgG Light Chain
SKU:
CABS061
Size:
100μL, 200μL, 50μL
Reactivity:
Rabbit
Clone Number:
AMC0531
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WBIP
Recommended Dilution:
WB
,1:5000 - 1:10000
Positive Sample:
Rabbit control IgG
Observed MW:
25kDa(Light chain)
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2864055
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (CABS061) at 1:10000 dilution. Lysates/proteins: 100 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg IKKα antibody (A2062' target='_blank' >A2062). Western blot was performed from the immunoprecipitate using IKKα antibody (A2062' target='_blank' >A2062) at a dilution of 1:1000. Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (CABS061) at 1:20000 dilution.
