Description
| Product Name: | HRP-SA (Streptavidin Horseradish Peroxidase) Activity ELISA Kit |
| SKU: | AEFI01231 |
| Size: | 96T |
| Reactivity: | Universal |
| Range: | 28.125-1800uIU/ml(1800uU=6ng) |
| Sensitivity: | 14uIU/ml |
| Detection Method: | Direct ELISA |
| Detection Wavelength: | OD450 |
| Reaction Duration: | 1 hours |
| Synonyms: | HRP-SA ELISA Kit, Streptavidin Horseradish Peroxidase ELISA Kit |
This kit was based on direct enzyme-linked immune-sorbent assay technology. Biotin was pre-coated onto 96-well plates. The
| Recovery: | Add a certain amount of SA-HRP into the sample. Calculate the recovery by comparing the measured value with the expected amount of SA-HRP in the sample.
| ||||||||||||||||||||||||||||||||||||||||||
| Linearity: | Dilute the sample with a certain amount of SA-HRP at 1:2, 1:4 and 1:8 to get the recovery range.
| ||||||||||||||||||||||||||||||||||||||||||
| Precision: | Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate. Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| ||||||||||||||||||||||||||||||||||||||||||
*Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Protocol |
| 1. | Wash the plate twice before adding the standard and sample. |
| 2. | Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C. |
| 3. | Washing: Aspirate and wash plates 5 times. |
| 4. | Add 90ul TMB Substrate Solution. Incubate 10-20 minutes at 37°C. |
| 5. | Add 50ul Stop Solution. Read at 450nm immediately and calculation. |
| Sample Type: | Quantitative detection of hrp-sa(horseradish peroxidase conjugated streptavidin) |
| Storage: | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity: | Specifically binds with SA-HRP , no obvious cross reaction with other analogues. |
