The HSP20/HSPB6 Monoclonal Antibody (CAB9091) is a high-quality antibody developed for reliable detection and analysis of target proteins. This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. RRID AB_2863655 Gene ID 126393 Swiss Prot Synonym HEL55; Hsp20; PPP1R91; HSP20/HSPB6
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSP20/HSPB6 Monoclonal Antibody
SKU:
CAB9091
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1787
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HEL55, Hsp20, PPP1R91, HSP20/HSPB6
Positive Sample:
Mouse heart, Rat skeletal muscle
Cellular Localization:
Cytoplasm, Nucleus, Translocates To Nuclear Foci During Heat Shock.
Calculated MW:
17kDa
Observed MW:
17kDa
This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. RRID AB_2863655 Gene ID 126393 Swiss Prot Synonym HEL55; Hsp20; PPP1R91; HSP20/HSPB6
Purification Method:
Affinity purification
Gene ID:
126393
RRID:
AB_2863655
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HSP20/HSPB6 Rabbit mAb (CAB9091) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using HSP20/HSPB6 Rabbit mAb (CAB9091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HSP20/HSPB6 Rabbit mAb (CAB9091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using HSP20/HSPB6 Rabbit mAb (CAB9091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using HSP20/HSPB6 Rabbit mAb (CAB9091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Rat heart tissue using HSP20/HSPB6 Rabbit mAb (CAB9091, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
