The HTR2C Polyclonal Antibody (CAB11831) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to HTR2C and is validated for use in Western blot applications. By targeting the HTR2C protein, this antibody allows for the detection and analysis of HTR2C in various cell types, making it an ideal choice for studies in neuroscience, psychiatry, and drug development.The HTR2C protein is a key player in the regulation of serotonin signaling in the brain, influencing a wide range of functions including mood, appetite, and sleep.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HTR2C Polyclonal Antibody
SKU:
CAB11831
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HTR1C, 5-HT1C, 5-HT2C, 5HTR2C, 5-HTR2C, HTR2C
Positive Sample:
SH-SY5Y, A-431, U-87MG, Jurkat, B cells
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
52kDa
Observed MW:
75kDa
This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants.
Purification Method
Affinity purification
Gene ID
3358
RRID
AB_2758798
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HTR2C Rabbit pAb (CAB11831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
