The HTRA2 Antibody (CAB14877) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit hosts, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications.HtrA2, also known as high temperature requirement protein A2, is a mitochondrial serine protease that plays a crucial role in regulating cell death and survival. Dysregulation of HtrA2 has been linked to various diseases, including neurodegenerative disorders and cancer.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HTRA2 Antibody
SKU:
CAB14877
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
27429
RRID
AB_2761757
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using HTRA2 Rabbit pAb (CAB14877) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg HTRA2 antibody (CAB14877). Western blot was performed from the immunoprecipitate using HTRA2 antibody (CAB14877) at a dilution of 1:1000.
