The Human 5'-Nucleotidase (Ecto-NT5E) ELISA Kit is a powerful tool for the precise measurement of ecto-5'-nucleotidase levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Ecto-5'-nucleotidase, also known as CD73, is an important enzyme involved in purinergic signaling and immune regulation. It plays a key role in processes such as immune response modulation, inflammation, and cell adhesion, making it a valuable biomarker for studying various diseases and potential therapeutic targets.
With the Human 5'-Nucleotidase (Ecto-NT5E) ELISA Kit, researchers can confidently analyze ecto-5'-nucleotidase levels in serum, plasma, and cell culture supernatants to advance their understanding of disease mechanisms and develop innovative treatments. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human 5'-Nucleotidase, Ecto (NT5E) ELISA Kit
Product Code:
HUDL02103
Size:
96 Assays
Target:
NT5E
Synonyms:
CD73, NT5-E, E5NT, E5-NT, NTE, EN, ENT
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
