The Human ACVB (Activin B) CLIA Kit is a reliable tool for accurately measuring activin B levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers consistent and reproducible results, facilitating various research applications.Activin B is a key protein involved in regulating various biological processes, including cell growth and differentiation. Dysregulation of activin B signaling has been implicated in diseases such as cancer, fibrosis, and inflammation, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions.
Overall, the Human ACVB CLIA Kit provides researchers with a valuable tool for studying activin B biology and its implications in disease pathology, helping advance our understanding of these complex biological processes.
Product Name:
Human ACVB (Activin B) CLIA Kit
SKU:
HUES00864
Target:
Human ACVB (Activin B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACVB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACVB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACVB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACVB. You can calculate the concentration of Human ACVB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
94-106
97-112
Average (%)
101
101
103
1:4
Range (%)
89-103
90-101
88-103
Average (%)
94
96
95
1:8
Range (%)
92-103
98-114
87-99
Average (%)
98
105
92
1:16
Range (%)
86-99
98-111
88-101
Average (%)
91
105
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.78
54.59
202.0
25.56
57.36
190.92
Standard deviation
2.37
6.16
22.52
2.63
5.6
13.44
C V (%)
9.97
11.28
11.15
10.29
9.76
7.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACVB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACVB in samples. No significant cross-reactivity or interference between Human ACVB and analogues was observed.
