Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) CLIA Kit
The Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of ADAMTS4 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.ADAMTS4 is a key enzyme involved in extracellular matrix degradation and has been implicated in various diseases such as osteoarthritis and cancer.
By measuring ADAMTS4 levels, researchers can better understand the role of this protein in disease progression and potentially identify new therapeutic targets.Overall, the Human ADAMTS4 CLIA Kit offers a valuable tool for studying the function of ADAMTS4 and its role in disease pathogenesis, ultimately leading to advancements in diagnostic and therapeutic strategies.
Product Name:
Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) CLIA Kit
SKU:
HUES00199
Target:
Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADAMTS4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAMTS4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAMTS4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADAMTS4. You can calculate the concentration of Human ADAMTS4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
90-106
88-101
Average (%)
99
97
95
1:4
Range (%)
88-103
99-114
90-101
Average (%)
94
106
96
1:8
Range (%)
101-115
98-113
90-105
Average (%)
109
106
96
1:16
Range (%)
96-111
99-115
98-111
Average (%)
103
105
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
105
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.56
133.21
498.45
44.76
134.87
472.87
Standard deviation
4.96
10.14
51.64
4.39
15.47
52.49
C V (%)
10.43
7.61
10.36
9.81
11.47
11.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADAMTS4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADAMTS4 in samples. No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.
