Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) ELISA Kit
The Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) ELISA Kit is a cutting-edge tool for the precise measurement of ADAMTS4 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures dependable and consistent results, making it an invaluable asset for a diverse array of research endeavors.
ADAMTS4 is a key enzyme involved in extracellular matrix remodeling and is implicated in various pathological processes such as inflammation, arthritis, and tissue damage. By accurately quantifying ADAMTS4 levels, researchers can gain valuable insights into these conditions and potentially identify novel therapeutic targets for intervention.
Product Name:
Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4) ELISA Kit
SKU:
HUES01503
Target:
Human ADAMTS4 (ADAM with Thrombospondin Type 1 Motif 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADAMTS4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAMTS4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAMTS4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADAMTS4. You can calculate the concentration of Human ADAMTS4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
93-105
89-104
Average (%)
100
99
95
1:4
Range (%)
88-102
88-99
85-100
Average (%)
94
93
91
1:8
Range (%)
87-101
86-98
85-97
Average (%)
93
93
91
1:16
Range (%)
87-102
83-96
84-95
Average (%)
93
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
95-106
101
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
194.77
637.05
1878.94
194.68
684.14
1824.55
Standard deviation
10.5
37.39
65.58
12.79
36.6
71.34
C V (%)
5.39
5.87
3.49
6.57
5.35
3.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADAMTS4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADAMTS4 in samples. No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.
