The Human Adiponectin Receptor 2 (AdipoR2) ELISA Kit is specifically designed for the accurate detection of AdipoR2 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.AdipoR2 is a key receptor involved in mediating the metabolic effects of adiponectin, an adipokine that plays a crucial role in regulating glucose and lipid metabolism. Dysregulation of the adiponectin signaling pathway is associated with metabolic disorders such as obesity, insulin resistance, and type 2 diabetes, making AdipoR2 a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its reliable performance and comprehensive coverage, the Human Adiponectin Receptor 2 (AdipoR2) ELISA Kit is an indispensable tool for researchers seeking to delve deeper into the intricate mechanisms of metabolic regulation and develop innovative strategies for managing metabolic disorders.
Product Name:
Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit
Product Code:
HUDL00082
Size:
96 Assays
Target:
ADIPOR2
Synonyms:
PAQR2, ACDCR2, Progestin and AdipoQ Receptor Family Member II
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
