The Human ADP/Acrp30 (Adiponectin) ELISA Kit is expertly designed for the precise quantitative detection of Adiponectin levels in various human biological samples. Adiponectin, a key adipokine, plays a critical role in regulating glucose levels and fatty acid breakdown, influencing insulin sensitivity and inflammation pathways. Its correlation with metabolic disorders, such as obesity and diabetes, underscores its significance in understanding metabolic health. Our ELISA kit ensures exceptional sensitivity and specificity, guaranteeing reliable and reproducible results in your research applications.
Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for investigating the role of Adiponectin in metabolic pathways and diseases. Trust Assay Genie's ADP/Acrp30 ELISA Kit for accurate quantification and comprehensive analysis of this important biomarker in your studies.
Product Name:
Human ADP/Acrp30 (Adiponectin) ELISA Kit
SKU:
AEES00151
Target:
Human ADP/Acrp30 (Adiponectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADP/Acrp30. You can calculate the concentration of Human ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-107
88-100
Average (%)
94
103
95
1:4
Range (%)
97-108
98-109
99-108
Average (%)
105
105
104
1:8
Range (%)
83-95
84-98
90-97
Average (%)
90
92
94
1:16
Range (%)
95-104
92-108
101-113
Average (%)
99
100
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
89-105
97
Cell culture media (n=5)
85-96
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.25
4.75
0.42
1.15
4.08
Standard deviation
0.03
0.06
0.25
0.02
0.05
0.2
C V (%)
6.25
4.8
5.26
4.76
4.35
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADP/Acrp30 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADP/Acrp30 in samples. No significant cross-reactivity or interference between Human ADP/Acrp30 and analogues was observed.
