Human ADP-HMW (High Molecular Weight Adiponectin) ELISA Kit
Based on the information provided in the URL, here is a product description:The Human ADP HMW (High Molecular Weight) Adiponectin ELISA Kit is specifically designed for the precise measurement of high molecular weight adiponectin levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent choice for a variety of research applications.High molecular weight adiponectin is a critical adipokine that plays a crucial role in regulating metabolism and insulin sensitivity.
Its levels have been linked to various metabolic disorders, including obesity, type 2 diabetes, and cardiovascular disease, making it a valuable biomarker for studying these conditions and evaluating potential therapeutic interventions. Overall, the Human ADP HMW Adiponectin ELISA Kit provides researchers with a reliable tool to investigate the role of high molecular weight adiponectin in metabolic and cardiovascular health, contributing to advancements in the understanding and treatment of related diseases.
Product Name:
Human ADP-HMW (High Molecular Weight Adiponectin) ELISA Kit
SKU:
HUES03592
Target:
Human ADP-HMW (High Molecular Weight Adiponectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADP-HMW. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADP-HMW and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP-HMW, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADP-HMW. You can calculate the concentration of Human ADP-HMW in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
95-111
93-106
Average (%)
94
103
99
1:4
Range (%)
91-106
81-91
86-100
Average (%)
99
86
91
1:8
Range (%)
86-98
83-97
84-97
Average (%)
93
88
89
1:16
Range (%)
93-108
81-91
82-95
Average (%)
99
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
96-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.25
60.03
153.92
18.1
55.36
155.6
Standard deviation
0.97
3.03
5.6
1.12
2.33
4.79
C V (%)
5.32
5.05
3.64
6.19
4.21
3.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADP-HMW concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADP-HMW in samples. No significant cross-reactivity or interference between Human ADP-HMW and analogues was observed.
