The Human AGC (Aggrecan) CLIA Kit is specifically designed for the precise measurement of Aggrecan levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it an essential tool for a variety of research applications.Aggrecan is a key component of cartilage tissue, playing a vital role in maintaining cartilage integrity and function.
Changes in Aggrecan levels have been implicated in various musculoskeletal disorders, such as osteoarthritis, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Human AGC CLIA Kit offers researchers a comprehensive solution for studying Aggrecan levels in human samples, contributing to a better understanding of cartilage biology and the development of novel treatments for musculoskeletal diseases.
Product Name:
Human AGC (Aggrecan) CLIA Kit
SKU:
HUES00215
Target:
Human AGC (Aggrecan)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AGC. You can calculate the concentration of Human AGC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
100-112
100-114
Average (%)
104
106
106
1:4
Range (%)
96-109
88-100
94-105
Average (%)
101
95
100
1:8
Range (%)
101-117
101-116
96-111
Average (%)
108
109
102
1:16
Range (%)
99-113
92-104
90-103
Average (%)
105
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.02
401.4
1442.71
209.36
371.69
1299.24
Standard deviation
25.98
37.61
148.02
20.16
44.08
87.18
C V (%)
12.31
9.37
10.26
9.63
11.86
6.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AGC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGC in samples. No significant cross-reactivity or interference between Human AGC and analogues was observed.
