Human AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit
The Human AGER (Advanced Glycosylation End Product-Specific Receptor) CLIA Kit is specifically designed for the accurate detection of AGER levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.AGER is a receptor involved in recognizing and binding to advanced glycosylation end products, which are formed during non-enzymatic reactions between sugars and proteins.
Dysregulation of the AGER pathway has been implicated in various diseases such as diabetes, inflammation, and aging-related conditions.By accurately measuring AGER levels, researchers can gain valuable insights into the role of this receptor in disease pathogenesis and progression. This kit provides a valuable tool for studying the AGER pathway and developing potential therapeutic interventions targeting this pathway.
Product Name:
Human AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit
SKU:
HUES00216
Target:
Human AGER (Advanced Glycosylation End Product Specific Receptor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGER. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AGER. You can calculate the concentration of Human AGER in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
101-114
92-106
Average (%)
106
108
98
1:4
Range (%)
94-106
91-104
103-116
Average (%)
100
97
109
1:8
Range (%)
100-116
99-114
91-104
Average (%)
109
107
98
1:16
Range (%)
97-113
97-114
85-99
Average (%)
104
104
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
97-109
104
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.11
157.18
401.39
46.72
155.27
398.69
Standard deviation
6.01
11.32
26.89
5.8
18.48
31.46
C V (%)
11.76
7.2
6.7
12.41
11.9
7.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AGER concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGER in samples. No significant cross-reactivity or interference between Human AGER and analogues was observed.
