The Human AGT (Angiotensinogen) CLIA Kit is a highly accurate and reliable assay designed for the detection of angiotensinogen levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Angiotensinogen is a key protein involved in the renin-angiotensin system, playing a crucial role in regulating blood pressure and fluid balance. Dysregulation of the renin-angiotensin system has been linked to cardiovascular diseases, kidney disorders, and hypertension, making angiotensinogen a valuable biomarker for understanding and potentially treating these conditions.
By using the Human AGT CLIA Kit, researchers can accurately measure angiotensinogen levels in human samples, providing valuable insights into the mechanisms underlying cardiovascular and kidney diseases. This kit is a valuable tool for studying the renin-angiotensin system and developing novel therapeutic strategies for related conditions.
Product Name:
Human AGT (Angiotensinogen) CLIA Kit
SKU:
HUES00220
Target:
Human AGT (Angiotensinogen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AGT. You can calculate the concentration of Human AGT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
90-103
87-101
Average (%)
106
97
94
1:4
Range (%)
89-103
89-101
102-114
Average (%)
95
94
108
1:8
Range (%)
84-95
90-105
89-103
Average (%)
90
97
95
1:16
Range (%)
86-102
87-102
102-118
Average (%)
93
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
100-115
108
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.83
7.58
0.92
2.68
6.97
Standard deviation
0.1
0.28
0.86
0.08
0.23
0.7
C V (%)
10.42
9.89
11.35
8.7
8.58
10.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AGT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGT in samples. No significant cross-reactivity or interference between Human AGT and analogues was observed.
