The Human AIM2 (Absent in Melanoma 2) CLIA Kit is specifically designed for the accurate measurement of AIM2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for various research applications.AIM2 is a critical protein involved in the innate immune response, particularly in detecting cytoplasmic DNA and activating inflammatory pathways. Dysregulation of AIM2 has been linked to various inflammatory diseases and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Human AIM2 CLIA Kit provides researchers with a reliable tool for analyzing AIM2 levels in human samples, contributing to a better understanding of the immune response and potential treatment strategies for related diseases. For more information and to purchase the kit, visit the link provided.
Product Name:
Human AIM2 (Absent In Melanoma 2) CLIA Kit
SKU:
HUES01267
Target:
Human AIM2 (Absent In Melanoma 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AIM2. You can calculate the concentration of Human AIM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
88-102
91-107
Average (%)
100
93
99
1:4
Range (%)
93-107
86-98
87-98
Average (%)
101
91
92
1:8
Range (%)
84-100
94-104
100-114
Average (%)
91
99
108
1:16
Range (%)
100-116
85-97
98-111
Average (%)
109
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
102-115
108
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.66
274.89
873.9
101.33
285.39
826.31
Standard deviation
8.37
29.41
57.42
10.8
28.62
65.61
C V (%)
8.23
10.7
6.57
10.66
10.03
7.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AIM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AIM2 in samples. No significant cross-reactivity or interference between Human AIM2 and analogues was observed.
