Human Alk-Smase (Alkaline Sphingomyelinase) CLIA Kit
The Human ALK-SMASE (alkaline sphingomyelinase) CLIA Kit is specifically designed for the precise measurement of ALK-SMASE levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications. ALK-SMASE is an enzyme that plays a critical role in sphingomyelin metabolism, which is essential for maintaining cell membrane integrity and signal transduction. Dysregulation of ALK-SMASE activity has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions.
Therefore, the detection of ALK-SMASE levels can provide valuable insights into disease mechanisms and potential therapeutic targets. By using the Human ALK-SMASE CLIA Kit, researchers can gain valuable information on the activity of this important enzyme, advancing our understanding of its role in health and disease. This kit is a valuable tool for studying sphingomyelin metabolism and its implications for various pathological conditions.
Product Name:
Human Alk-Smase (Alkaline Sphingomyelinase) CLIA Kit
SKU:
HUES00225
Target:
Human Alk-Smase (Alkaline Sphingomyelinase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Alk-Smase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Alk-Smase, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Alk-Smase. You can calculate the concentration of Human Alk-Smase in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
104-116
88-104
Average (%)
95
110
95
1:4
Range (%)
99-115
91-107
87-99
Average (%)
108
99
92
1:8
Range (%)
86-99
95-109
99-113
Average (%)
91
101
107
1:16
Range (%)
86-100
94-107
91-106
Average (%)
91
100
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
237.78
485.98
2153.04
259.83
511.81
1965.23
Standard deviation
20.95
44.37
168.15
27.05
45.86
136.78
C V (%)
8.81
9.13
7.81
10.41
8.96
6.96
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Alk-Smase concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human Alk-Smase in samples. No significant cross-reactivity or interference between Human Alk-Smase and analogues was observed.
