The Human Alpha-Lactalbumin CLIA Kit is specifically designed for the precise measurement of alpha-lactalbumin levels in human biological samples including serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Alpha-lactalbumin is a vital protein that plays a crucial role in lactation and infant nutrition.
It is also known for its potential anticancer and antiviral properties, making it a valuable biomarker for studying various health conditions and developing targeted therapies. With the Human Alpha-Lactalbumin CLIA Kit, researchers can easily detect and quantify alpha-lactalbumin levels, advancing their understanding of its physiological functions and potential therapeutic applications.
Product Name:
Human alpha-La (Alpha-Lactalbumin) CLIA Kit
SKU:
HUES00580
Target:
Human alpha-La (Alpha-Lactalbumin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human α-La. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human α-La and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human α-La, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human α-La. You can calculate the concentration of Human α-La in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
101-114
103-118
Average (%)
95
108
110
1:4
Range (%)
100-115
91-106
94-107
Average (%)
107
97
101
1:8
Range (%)
102-113
88-98
103-121
Average (%)
107
93
110
1:16
Range (%)
87-97
101-113
100-116
Average (%)
92
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
102-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.56
4.95
0.59
1.65
5.4
Standard deviation
0.05
0.14
0.41
0.08
0.13
0.57
C V (%)
9.09
8.97
8.28
13.56
7.88
10.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human α-La concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human α-La in samples. No significant cross-reactivity or interference between Human α-La and analogues was observed.
