Human alpha1-AGP (Alpha-1-Acid Glycoprotein) CLIA Kit
The Human Alpha-1 AGP (Alpha-1 Acid Glycoprotein) CLIA Kit is specifically designed for the accurate detection of alpha-1 acid glycoprotein levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing dependable and consistent results for a diverse array of research applications.Alpha-1 acid glycoprotein is a vital glycoprotein that plays a critical role in inflammation, immune response, and drug metabolism. Its levels have been linked to various disease states, including inflammatory conditions, infections, and certain types of cancer.
As a result, this kit serves as a valuable tool for investigating these conditions and potentially uncovering new therapeutic options.Overall, the Human Alpha-1 AGP CLIA Kit is an indispensable resource for researchers looking to accurately measure and analyze alpha-1 acid glycoprotein levels in biological samples, paving the way for advancements in biomedical research and clinical diagnostics.
Product Name:
Human alpha1-AGP (Alpha-1-Acid Glycoprotein) CLIA Kit
SKU:
HUES00001
Target:
Human alpha1-AGP (Alpha-1-Acid Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AGP1. You can calculate the concentration of Human AGP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-120
103-116
94-107
Average (%)
109
108
101
1:4
Range (%)
93-108
101-114
91-104
Average (%)
100
107
98
1:8
Range (%)
85-98
89-102
87-101
Average (%)
90
96
93
1:16
Range (%)
98-111
90-102
88-99
Average (%)
104
96
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-111
106
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
100-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.51
4.56
18.74
2.62
4.79
18.41
Standard deviation
0.28
0.44
1.65
0.25
0.54
1.97
C V (%)
11.16
9.65
8.8
9.54
11.27
10.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AGP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGP1 in samples. No significant cross-reactivity or interference between Human AGP1 and analogues was observed.
