Human alphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit
The Human Alpha-MSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit is specifically designed for the accurate and sensitive detection of Alpha-MSH levels in human serum, plasma, and cell culture supernatants. This kit offers high precision and reliability, ensuring consistent and reproducible results for a variety of research applications.Alpha-MSH is a key hormone involved in regulating skin pigmentation and immune response. It also plays a role in appetite regulation, energy balance, and inflammation.
Abnormal levels of Alpha-MSH have been associated with conditions such as skin disorders, obesity, and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. By using the Human Alpha-MSH ELISA Kit, researchers can accurately measure Alpha-MSH levels in biological samples with confidence, helping to advance our understanding of its role in various physiological and pathological processes.
Product Name:
Human alphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit
SKU:
HUES03283
Target:
Human alphaMSH (Alpha-Melanocyte Stimulating Hormone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with αMSH. During the reaction, αMSH in the sample or standard competes with a fixed amount of αMSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to αMSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of αMSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
87-100
98-110
Average (%)
95
92
103
1:4
Range (%)
84-97
93-107
93-106
Average (%)
90
98
99
1:8
Range (%)
86-100
92-105
93-110
Average (%)
92
100
100
1:16
Range (%)
83-98
88-100
100-114
Average (%)
90
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.9
4.7
0.5
0.9
4.5
Standard deviation
0.04
0.04
0.22
0.03
0.04
0.15
C V (%)
8
4.44
4.68
6.0
4.44
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of αMSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes αMSH in samples. No significant cross-reactivity or interference between αMSH and analogues was observed.
