Human alphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit
The Human Alpha-MSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit is specifically designed for the accurate and sensitive detection of Alpha-MSH levels in human serum, plasma, and cell culture supernatants. This kit offers high precision and reliability, ensuring consistent and reproducible results for a variety of research applications.Alpha-MSH is a key hormone involved in regulating skin pigmentation and immune response. It also plays a role in appetite regulation, energy balance, and inflammation. Abnormal levels of Alpha-MSH have been associated with conditions such as skin disorders, obesity, and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. By using the Human Alpha-MSH ELISA Kit, researchers can accurately measure Alpha-MSH levels in biological samples with confidence, helping to advance our understanding of its role in various physiological and pathological processes.
Product Name:
Human alphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit
SKU:
HUES03283
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
