Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) CLIA Kit
The Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) CLIA Kit is a reliable and efficient tool for the accurate measurement of AMBP levels in human serum, plasma, and cell culture supernatants. This kit is specifically designed to provide high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.AMBP, also known as Alpha-1-Microglobulin/Bikunin Precursor, is a multifunctional protein involved in various physiological processes, including the regulation of inflammation and oxidative stress.
Abnormal levels of AMBP have been linked to conditions such as kidney diseases, sepsis, and diabetes, making it a valuable biomarker for studying these diseases and identifying potential therapeutic targets.By using the Human AMBP CLIA Kit, researchers can accurately measure AMBP levels in clinical samples, allowing for a better understanding of its role in disease pathogenesis and the development of novel diagnostic and therapeutic strategies.
Product Name:
Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) CLIA Kit
SKU:
HUES00229
Target:
Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AMBP/α1M. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMBP/α1M and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMBP/α1M, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AMBP/α1M. You can calculate the concentration of Human AMBP/α1M in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
87-99
100-116
Average (%)
98
93
108
1:4
Range (%)
96-113
92-102
99-112
Average (%)
104
97
105
1:8
Range (%)
96-110
94-108
103-118
Average (%)
103
100
109
1:16
Range (%)
99-115
98-111
102-115
Average (%)
106
105
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
101
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.92
9.19
32.67
4.24
9.6
32.28
Standard deviation
0.42
0.96
2.57
0.41
1.09
2.69
C V (%)
10.71
10.45
7.87
9.67
11.35
8.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AMBP/α1M concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AMBP/α1M in samples. No significant cross-reactivity or interference between Human AMBP/α1M and analogues was observed.
