Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
The Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit is specifically designed for the precise detection of AMBP levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.AMBP, also known as Alpha-1-Microglobulin/Bikunin Precursor, is a key protein involved in various physiological processes such as inflammation, tissue repair, and immune response modulation.
Dysregulation of AMBP levels has been associated with conditions like kidney disease, sepsis, and certain cancers, underscoring its importance as a potential biomarker for disease diagnosis and therapeutic development.With its reliable performance and versatility, the Human AMBP ELISA Kit is an essential tool for researchers investigating the role of AMBP in health and disease. Trust in this kit to deliver accurate and valuable insights into the complex functions of AMBP in the human body.
Product Name:
Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
SKU:
HUES01539
Target:
Human AMBP (Alpha-1-Microglobulin/Bikunin Precursor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
2.81 ng/mL
Detection range:
4.69-300 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AMBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AMBP. You can calculate the concentration of Human AMBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
91-107
88-100
Average (%)
102
99
94
1:4
Range (%)
85-99
80-94
88-102
Average (%)
92
87
94
1:8
Range (%)
86-99
87-97
86-98
Average (%)
92
92
93
1:16
Range (%)
90-105
78-90
90-101
Average (%)
98
85
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
15.67
34.52
126.73
15.18
37.27
130.68
Standard deviation
1.06
1.66
6.37
1.01
2.16
5.89
C V (%)
6.76
4.81
5.03
6.65
5.8
4.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AMBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AMBP in samples. No significant cross-reactivity or interference between Human AMBP and analogues was observed.
