The Human ANG1 (Angiopoietin-1) CLIA Kit is specifically designed to accurately measure levels of Angiopoietin-1 in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Angiopoietin-1 is a key protein involved in angiogenesis, the process of forming new blood vessels. It plays a crucial role in regulating blood vessel development and stabilization, making it a valuable biomarker for studying various conditions such as cancer, cardiovascular diseases, and neurological disorders.
Understanding the levels of Angiopoietin-1 in different biological samples can provide important insights for research and potential therapeutic interventions.Overall, the Human ANG1 CLIA Kit from Assaygenie is an essential tool for researchers looking to accurately measure Angiopoietin-1 levels and further explore its role in various physiological and pathological processes.
Product Name:
Human ANG1 (Angiopoietin 1) CLIA Kit
SKU:
HUES00007
Target:
Human ANG1 (Angiopoietin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANG1. You can calculate the concentration of Human ANG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
85-97
90-102
Average (%)
100
90
95
1:4
Range (%)
95-109
88-101
90-107
Average (%)
101
95
98
1:8
Range (%)
92-107
89-104
94-108
Average (%)
99
95
101
1:16
Range (%)
100-117
103-119
96-110
Average (%)
107
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
98
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.07
89.3
448.85
56.1
90.66
441.97
Standard deviation
5.22
8.96
45.92
4.55
6.96
47.38
C V (%)
10.22
10.03
10.23
8.11
7.68
10.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG1 in samples. No significant cross-reactivity or interference between Human ANG1 and analogues was observed.
