The Human Angiopoietin 1 (ANG1) ELISA Kit is meticulously engineered for the precise and quantitative detection of Angiopoietin 1 levels in diverse biological samples of human origin. Angiopoietin 1 is a critical factor involved in regulating vascular development, endothelial cell function, and angiogenesis, offering valuable insights into vascular homeostasis and angiogenic processes.
Our ELISA kit provides exceptional sensitivity and specificity to ensure accurate and reproducible results in your research endeavors. With stringent adherence to quality control standards, this kit delivers robust performance and user-friendly protocols, making it an indispensable tool for exploring the role of ANG1 in various physiological and pathological conditions. Trust Assay Genie's ELISA kit for dependable quantification and comprehensive analysis of Angiopoietin 1 biomarkers in your studies.
Product Name:
Human ANG1 (Angiopoietin 1) ELISA Kit
SKU:
AEES00149
Target:
Human ANG1 (Angiopoietin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANG1. You can calculate the concentration of Human ANG1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
89-99
96-109
Average (%)
102
93
101
1:4
Range (%)
97-107
85-98
87-96
Average (%)
104
93
93
1:8
Range (%)
102-112
100-112
95-109
Average (%)
105
104
103
1:16
Range (%)
87-100
95-105
87-95
Average (%)
93
100
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-101
98
EDTA plasma (n=5)
96-107
100
Cell culture media (n=5)
88-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
173.65
486.98
1713.67
181.05
446.78
1717.07
Standard deviation
10.07
22.35
74.89
10.79
21.94
92.03
C V (%)
5.8
4.59
4.37
5.96
4.91
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANG1 in samples. No significant cross-reactivity or interference between Human ANG1 and analogues was observed.
