Human ANGPTL1 (Angiopoietin Like Protein 1) CLIA Kit
The Human ANGPTL1 (Angiopoietin-Like Protein 1) CLIA Kit is a powerful tool for the quantitative measurement of ANGPTL1 levels in human samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers superior sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ANGPTL1 is a key factor in regulating lipid metabolism, insulin sensitivity, and angiogenesis. Dysregulation of ANGPTL1 has been implicated in various diseases including obesity, diabetes, and cardiovascular disorders.
Therefore, accurate measurement of ANGPTL1 levels is crucial for understanding the mechanisms underlying these conditions and developing targeted therapies.With its reliable performance and broad applicability, the Human ANGPTL1 CLIA Kit is an essential tool for researchers studying the role of ANGPTL1 in health and disease. Order yours today to advance your research efforts in the field of angiogenesis and metabolic disorders.
Product Name:
Human ANGPTL1 (Angiopoietin Like Protein 1) CLIA Kit
SKU:
HUES00236
Target:
Human ANGPTL1 (Angiopoietin Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANGPTL1. You can calculate the concentration of Human ANGPTL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
101-114
102-115
Average (%)
98
107
109
1:4
Range (%)
102-115
98-114
98-112
Average (%)
108
104
106
1:8
Range (%)
91-105
102-116
95-110
Average (%)
97
110
100
1:16
Range (%)
86-101
98-114
91-105
Average (%)
92
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.95
178.13
992.75
111.18
176.64
1033.93
Standard deviation
10.46
17.78
75.75
12.39
17.89
117.45
C V (%)
10.16
9.98
7.63
11.14
10.13
11.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANGPTL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL1 in samples. No significant cross-reactivity or interference between Human ANGPTL1 and analogues was observed.
