Human ANGPTL4 (Angiopoietin Like Protein 4) CLIA Kit
The Human ANGPTL4 (Angiopoietin-like protein 4) CLIA Kit is specially designed for the quantitative measurement of ANGPTL4 levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity, specificity, and accuracy, ensuring precise and reliable results for a variety of research applications.ANGPTL4 is a key protein involved in regulating lipid metabolism, inflammation, and angiogenesis. It has been implicated in various diseases including obesity, cardiovascular diseases, and cancer, making it a valuable biomarker for studying these conditions and developing novel therapeutic strategies.
With easy-to-follow protocols and quick results, the Human ANGPTL4 CLIA Kit from Assay Genie is an essential tool for researchers looking to investigate the role of ANGPTL4 in health and disease. Reach new insights and advance your research with this advanced kit.
Product Name:
Human ANGPTL4 (Angiopoietin Like Protein 4) CLIA Kit
SKU:
HUES00239
Target:
Human ANGPTL4 (Angiopoietin Like Protein 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANGPTL4. You can calculate the concentration of Human ANGPTL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
86-98
95-112
Average (%)
92
91
102
1:4
Range (%)
99-115
89-99
87-99
Average (%)
106
94
93
1:8
Range (%)
95-110
87-101
94-111
Average (%)
102
93
101
1:16
Range (%)
99-115
100-114
99-116
Average (%)
105
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
1.96
7.25
0.99
2.01
7.6
Standard deviation
0.14
0.23
0.68
0.11
0.18
0.49
C V (%)
12.73
11.73
9.38
11.11
8.96
6.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANGPTL4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL4 in samples. No significant cross-reactivity or interference between Human ANGPTL4 and analogues was observed.
