Human ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit
The Human ANGPTL4 (Angiopoietin-like protein 4) ELISA Kit is specifically designed for the accurate quantification of ANGPTL4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.ANGPTL4 is a critical protein involved in the regulation of lipid metabolism, insulin sensitivity, and inflammation. Its dysregulation has been linked to various metabolic disorders, including obesity, diabetes, and cardiovascular diseases.
As such, the measurement of ANGPTL4 levels can provide valuable insights into the development and progression of these conditions, making it a valuable tool for researchers and clinicians alike.Overall, the Human ANGPTL4 ELISA Kit is an essential resource for studying the role of ANGPTL4 in metabolism and disease pathogenesis, offering a reliable and efficient method for biomarker detection and analysis.
Product Name:
Human ANGPTL4 (Angiopoietin Like Protein 4) ELISA Kit
SKU:
HUES01556
Target:
Human ANGPTL4 (Angiopoietin Like Protein 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ANGPTL4. You can calculate the concentration of Human ANGPTL4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
98-113
87-101
Average (%)
94
103
92
1:4
Range (%)
90-104
85-97
90-104
Average (%)
97
90
95
1:8
Range (%)
91-105
85-98
87-99
Average (%)
98
90
93
1:16
Range (%)
89-99
85-97
84-99
Average (%)
94
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.73
12.85
40.94
4.48
12.46
40.23
Standard deviation
0.29
0.57
2.04
0.28
0.52
1.62
C V (%)
6.13
4.44
4.98
6.25
4.17
4.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ANGPTL4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL4 in samples. No significant cross-reactivity or interference between Human ANGPTL4 and analogues was observed.
