Human ANGPTL6 (Angiopoietin Like Protein 6) CLIA Kit
The Human ANGPTL6 (Angiopoietin-like Protein 6) CLIA Kit is specifically designed for the precise measurement of ANGPTL6 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for a variety of research purposes.ANGPTL6 is a key protein involved in regulating lipid metabolism and insulin sensitivity, making it a crucial player in metabolic disorders such as obesity and diabetes.
By accurately measuring ANGPTL6 levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially uncover new therapeutic targets.Overall, the Human ANGPTL6 CLIA Kit offers researchers a reliable and efficient method for studying the role of ANGPTL6 in metabolic disorders and advancing research in the field of lipid metabolism and insulin regulation.
Product Name:
Human ANGPTL6 (Angiopoietin Like Protein 6) CLIA Kit
SKU:
HUES00240
Target:
Human ANGPTL6 (Angiopoietin Like Protein 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ANGPTL6. You can calculate the concentration of Human ANGPTL6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
91-106
94-106
Average (%)
109
97
100
1:4
Range (%)
102-118
90-105
102-119
Average (%)
108
96
109
1:8
Range (%)
102-116
87-101
99-112
Average (%)
108
93
106
1:16
Range (%)
98-111
89-102
91-104
Average (%)
104
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-112
106
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.11
1.8
9.65
1.09
1.66
8.79
Standard deviation
0.1
0.19
0.98
0.09
0.19
0.76
C V (%)
9.01
10.56
10.16
8.26
11.45
8.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ANGPTL6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ANGPTL6 in samples. No significant cross-reactivity or interference between Human ANGPTL6 and analogues was observed.
