The Human AP13 (Apelin-13) ELISA Kit is specially designed for the precise measurement of apelin-13 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent choice for various research purposes.Apelin-13 is a key peptide involved in regulating cardiovascular functions, including blood pressure regulation, cardiac contractility, and vasodilation.
Its dysregulation has been linked to several cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With the Human AP13 (Apelin-13) ELISA Kit, researchers can confidently evaluate apelin-13 levels in biological samples, gaining valuable insights into its physiological and pathological roles.Upgrade your research with this reliable and efficient ELISA kit from Assay Genie.
Product Name:
Human AP13 (Apelin 13) ELISA Kit
SKU:
HUES01663
Target:
Human AP13 (Apelin 13)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with AP13. During the reaction, AP13 in the sample or standard competes with a fixed amount of AP13 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to AP13. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AP13 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
97-111
94-105
Average (%)
103
103
100
1:4
Range (%)
86-97
90-102
98-114
Average (%)
91
97
104
1:8
Range (%)
82-96
91-104
101-112
Average (%)
89
97
106
1:16
Range (%)
85-97
89-105
97-115
Average (%)
91
96
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
426.7
1074.7
3419.8
465.1
1177.9
3157.7
Standard deviation
28.2
51.6
143.6
31.6
56.5
132.6
C V (%)
6.61
4.8
4.2
6.79
4.8
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of AP13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes AP13 in samples. No significant cross-reactivity or interference between AP13 and analogues was observed.
