The Human ApoA5 (Apolipoprotein A5) ELISA Kit is meticulously crafted for the precise quantitative analysis of ApoA5 levels in various human biological samples. Apolipoprotein A5 is a key regulator of lipid metabolism, particularly in triglyceride-rich lipoproteins, playing a pivotal role in the modulation of plasma triglyceride levels. Accurate measurement of ApoA5 is essential for investigating its role in lipid metabolism and understanding its implications in cardiovascular health, metabolic diseases, and lipid-related disorders.
This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. With robust performance and stringent quality control measures, this kit is a reliable choice for research and clinical applications. Trust in the Assay Genie's ApoA5 ELISA Kit for precise and dependable quantification of this important biomarker in your studies.
Product Name:
Human ApoA5 (Apolipoprotein A5) ELISA Kit
SKU:
AEES00077
Target:
Human ApoA5 (Apolipoprotein A5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoA5. You can calculate the concentration of Human ApoA5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-95
97-106
84-97
Average (%)
90
102
91
1:4
Range (%)
87-101
101-107
90-102
Average (%)
93
104
95
1:8
Range (%)
97-108
86-97
102-113
Average (%)
105
93
105
1:16
Range (%)
84-96
87-96
91-101
Average (%)
90
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
100-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
168.97
447.94
1846.03
177.36
498.83
1844.5
Standard deviation
10.14
22.4
82.33
9.08
22.6
95.55
C V (%)
6
5.0
4.46
5.12
4.53
5.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoA5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed.
