The Human ApoB48 (Apolipoprotein B48) ELISA Kit is specifically developed for the precise and quantitative detection of Apolipoprotein B48 levels in a variety of human biological samples. Apolipoprotein B48 is a vital protein involved in lipid metabolism and transport, particularly in transport chylomicrons after feeding. Accurate measurement of ApoB48 plays a significant role in understanding lipoprotein metabolism, particularly in the postprandial state, and its implications in cardiovascular health and disease.
Our ELISA kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results in assessing ApoB48 concentrations. Manufactured under stringent quality control measures, this kit delivers robust performance and ease of use, making it an ideal choice for both research and clinical purposes. Count on Assay Genie's Human ApoB48 ELISA Kit for reliable and consistent quantification of this essential biomarker in your investigations.
Product Name:
Human ApoB48 (Apolipoprotein B48) ELISA Kit
SKU:
AEES00120
Target:
Human ApoB48 (Apolipoprotein B48)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoB48. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoB48 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoB48, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoB48. You can calculate the concentration of Human ApoB48 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-107
89-100
100-107
Average (%)
102
93
103
1:4
Range (%)
88-94
85-95
97-111
Average (%)
91
92
105
1:8
Range (%)
98-112
98-106
100-107
Average (%)
105
103
103
1:16
Range (%)
97-104
88-99
88-98
Average (%)
101
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-99
95
EDTA plasma (n=5)
91-99
95
Cell culture media (n=5)
85-93
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.52
10.09
46.17
4.65
11.98
40.55
Standard deviation
0.27
0.48
2.18
0.24
0.56
1.73
C V (%)
5.97
4.76
4.72
5.16
4.67
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ApoB48 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ApoB48 in samples. No significant cross-reactivity or interference between Human ApoB48 and analogues was observed.
