The Human ARG1 (Arginase I) ELISA Kit is a highly reliable and sensitive tool for the quantification of arginase I levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it well-suited for a variety of research applications.Arginase I is an enzyme that plays a key role in the urea cycle and is involved in the metabolism of arginine. Dysregulation of arginase I has been linked to various diseases including cancer, cardiovascular disorders, and immune system dysfunction.
Thus, measuring arginase I levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of potential therapies.Overall, the Human ARG1 ELISA Kit offers researchers a powerful tool for studying the role of arginase I in health and disease, with the precision and reliability needed for meaningful scientific discoveries.
Product Name:
Human ARG1 (Arginase I) ELISA Kit
SKU:
HUES01696
Target:
Human ARG1 (Arginase I)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ARG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ARG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ARG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ARG1. You can calculate the concentration of Human ARG1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
92-104
95-106
Average (%)
101
98
100
1:4
Range (%)
86-98
82-95
88-99
Average (%)
93
87
93
1:8
Range (%)
90-104
86-98
83-95
Average (%)
98
92
89
1:16
Range (%)
89-103
87-99
83-94
Average (%)
94
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.94
24.52
94.93
11.33
25.17
100.73
Standard deviation
0.7
1.21
2.94
0.7
1.35
4.39
C V (%)
6.4
4.93
3.1
6.18
5.36
4.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ARG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ARG1 in samples. No significant cross-reactivity or interference between Human ARG1 and analogues was observed.
