The Human AST (Aspartate Aminotransferase) ELISA Kit is specifically designed for the precise detection of AST levels in human serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring consistent and accurate results, making it perfect for a variety of research purposes.AST is an important enzyme that plays a key role in amino acid metabolism and is primarily found in the liver, heart, and skeletal muscle.
Changes in AST levels can indicate liver disease, heart damage, or muscle injury, making it a valuable biomarker for monitoring and diagnosing these conditions.With the Human AST ELISA Kit, researchers can gain valuable insights into various health conditions and potentially develop targeted treatments to improve patient outcomes. Elevate your research with this reliable and innovative ELISA kit today.
Product Name:
Human AST (Aspartate Aminotransferase) ELISA Kit
SKU:
HUES01711
Target:
Human AST (Aspartate Aminotransferase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AST. You can calculate the concentration of Human AST in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
88-101
84-97
Average (%)
102
94
90
1:4
Range (%)
88-101
82-96
84-99
Average (%)
94
88
90
1:8
Range (%)
87-101
87-99
85-95
Average (%)
94
92
90
1:16
Range (%)
93-109
86-101
86-99
Average (%)
99
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
93-103
98
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.88
7.87
0.95
2.76
7.3
Standard deviation
0.07
0.17
0.26
0.05
0.14
0.25
C V (%)
7.45
5.9
3.3
5.26
5.07
3.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AST in samples. No significant cross-reactivity or interference between Human AST and analogues was observed.
