Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit
The Human B Lymphocyte Chemoattractant-1 (BLC1) ELISA Kit is a powerful tool for precise measurement of BLC1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an ideal choice for various research applications.BLC1, also known as CXCL13, is a chemokine that plays a vital role in immune response by attracting B lymphocytes to specific sites within the body.
It is involved in the formation and function of lymphoid tissues and is associated with various immune-related disorders.This ELISA kit provides researchers with a valuable tool for studying the role of BLC1 in immune responses, inflammation, and autoimmune diseases. By accurately measuring BLC1 levels, researchers can gain insights into potential therapeutic targets and biomarkers for various conditions.
Product Name:
Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit
Product Code:
HUDL00333
Size:
96 Assays
Target:
BLC1
Synonyms:
CXCL13, SCYB13, BLC, BCA1, BLR1L, ANGIE, ANGIE2, Chemokine(C-X-C-Motif)ligand 13, Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
