Human Baculoviral IAP Repeat Containing Protein 6 (BIRC6) ELISA Kit
The Human Baculoviral IAP repeat-containing protein 6 (BIRC6) ELISA Kit is a powerful tool for the accurate measurement of BIRC6 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it invaluable for a variety of research purposes.BIRC6 is a critical protein involved in various cellular processes, including apoptosis regulation, cell cycle control, and DNA repair. Dysregulation of BIRC6 has been linked to numerous diseases, including cancer, autoimmune disorders, and neurodegenerative conditions, highlighting its importance as a potential biomarker for diagnostic and therapeutic applications.
By utilizing the Human BIRC6 ELISA Kit, researchers can gain valuable insights into the role of this protein in disease development and progression, paving the way for the development of novel treatment strategies and diagnostic tools. Trust in the accuracy and reliability of this kit to advance your research efforts in the field of BIRC6 biology.
Product Name:
Human Baculoviral IAP Repeat Containing Protein 6 (BIRC6) ELISA Kit
Product Code:
HUDL00330
Size:
96 Assays
Target:
BIRC6
Synonyms:
BRUCE, Apollon, BIR repeat-containing ubiquitin-conjugating enzyme, Ubiquitin-conjugating BIR domain enzyme apollon
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
