Human BCL2L1/BCL-X (Bcl-2 Like Protein 1/Bcl2 Associated X Protein) CLIA Kit
The Human BCL2L1 (BCL-x, BCL-2-like protein 1) ELISA Kit is specifically designed for the accurate measurement of BCL2L1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.BCL2L1 is a key protein involved in regulating cell survival and apoptosis, making it a crucial factor in various diseases including cancer, neurodegenerative disorders, and autoimmune conditions.
Understanding the levels of BCL2L1 in biological samples can provide valuable insights into disease mechanisms and potential therapeutic strategies.Get reliable and precise results with the Human BCL2L1 ELISA Kit, a valuable tool for studying the role of BCL2L1 in health and disease.
Product Name:
Human BCL2L1/BCL-X (Bcl-2 Like Protein 1/Bcl2 Associated X Protein) CLIA Kit
SKU:
HUES00378
Target:
Human BCL2L1/BCL-X (Bcl-2 Like Protein 1/Bcl2 Associated X Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BCL2L1/BCL-X. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BCL2L1/BCL-X and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BCL2L1/BCL-X, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BCL2L1/BCL-X. You can calculate the concentration of Human BCL2L1/BCL-X in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
98-116
94-106
Average (%)
101
106
99
1:4
Range (%)
99-111
102-117
86-98
Average (%)
105
107
91
1:8
Range (%)
96-108
88-100
85-99
Average (%)
103
93
92
1:16
Range (%)
89-105
93-109
98-113
Average (%)
96
99
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.44
392.4
1521.8
200.97
380.04
1385.58
Standard deviation
22.21
27.62
151.42
25.22
35.69
85.77
C V (%)
10.81
7.04
9.95
12.55
9.39
6.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BCL2L1/BCL-X concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BCL2L1/BCL-X in samples. No significant cross-reactivity or interference between Human BCL2L1/BCL-X and analogues was observed.
