The Human BCL2L11 (BCL-2 Like Protein 11) CLIA Kit is specifically designed for the precise measurement of BCL2L11 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity to ensure accurate and consistent results, making it an invaluable tool for a variety of research applications.BCL2L11, also known as BIM, is a critical protein involved in the regulation of apoptosis and cell survival. Dysregulation of BCL2L11 has been implicated in various diseases, including cancer, autoimmune disorders, and neurological conditions.
As a result, BCL2L11 serves as an important biomarker for studying these diseases and exploring potential therapeutic interventions.With its reliable performance and broad utility, the Human BCL2L11 CLIA Kit is essential for researchers aiming to explore the role of BCL2L11 in health and disease, providing valuable insights into disease mechanisms and potential treatment strategies.
Product Name:
Human Bcl2L11 (Bcl-2 Like Protein 11) CLIA Kit
SKU:
HUES00391
Target:
Human Bcl2L11 (Bcl-2 Like Protein 11)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Bcl2L11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Bcl2L11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Bcl2L11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Bcl2L11. You can calculate the concentration of Human Bcl2L11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
90-103
92-107
Average (%)
100
98
98
1:4
Range (%)
84-96
89-106
86-100
Average (%)
91
97
92
1:8
Range (%)
92-106
87-101
93-108
Average (%)
98
94
99
1:16
Range (%)
85-96
88-100
85-99
Average (%)
90
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.9
415.89
1938.17
222.3
409.0
1846.81
Standard deviation
27.87
36.52
218.43
26.12
45.52
205.73
C V (%)
12.56
8.78
11.27
11.75
11.13
11.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Bcl2L11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Bcl2L11 in samples. No significant cross-reactivity or interference between Human Bcl2L11 and analogues was observed.
