The Human Beta-CTX (Beta Crosslaps) ELISA Kit is specifically designed for the precise measurement of beta-crosslaps levels in human serum and plasma samples. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.Beta-crosslaps are fragments of type I collagen that serve as markers for bone resorption, making them valuable indicators of bone turnover and health. Elevated levels of beta-crosslaps have been associated with conditions such as osteoporosis, hyperparathyroidism, and Paget's disease, highlighting their significance in diagnosing and monitoring bone-related disorders.
By utilizing the Human Beta-CTX ELISA Kit, researchers can better understand the mechanisms of bone metabolism and assess the effectiveness of treatments aimed at improving bone health. This kit provides a valuable tool for investigating bone-related diseases and developing targeted therapies to address them effectively.
Product Name:
Human beta-CTx (Beta Crosslaps) ELISA Kit
SKU:
HUES02082
Target:
Human beta-CTx (Beta Crosslaps)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-CTx. During the reaction, β-CTx in the sample or standard competes with a fixed amount of β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
93-107
87-98
Average (%)
92
101
92
1:4
Range (%)
86-101
92-107
100-113
Average (%)
93
99
106
1:8
Range (%)
90-103
89-102
94-108
Average (%)
96
96
102
1:16
Range (%)
87-103
87-100
95-109
Average (%)
94
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
399.6
1081.0
2908.7
419.0
1051.0
2875.8
Standard deviation
26.8
62.7
130.9
23.9
49.4
158.2
C V (%)
6.71
5.8
4.5
5.7
4.7
5.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of β-CTx concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes β-CTx in samples. No significant cross-reactivity or interference between β-CTx and analogues was observed.
