The Human Beta-CTX (Beta Crosslaps) ELISA Kit is specifically designed for the precise measurement of beta-crosslaps levels in human serum and plasma samples. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.Beta-crosslaps are fragments of type I collagen that serve as markers for bone resorption, making them valuable indicators of bone turnover and health. Elevated levels of beta-crosslaps have been associated with conditions such as osteoporosis, hyperparathyroidism, and Paget's disease, highlighting their significance in diagnosing and monitoring bone-related disorders. By utilizing the Human Beta-CTX ELISA Kit, researchers can better understand the mechanisms of bone metabolism and assess the effectiveness of treatments aimed at improving bone health. This kit provides a valuable tool for investigating bone-related diseases and developing targeted therapies to address them effectively.
Product Name:
Human beta-CTx (Beta Crosslaps) ELISA Kit
SKU:
HUES02082
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.