Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
The Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit is specifically designed for the quantitative assessment of bFGF/FGF2 levels in various human biological samples. Basic fibroblast growth factor, also known as FGF2, plays a fundamental role in cell growth, wound healing, and tissue regeneration processes. As a potent angiogenic factor, bFGF/FGF2 is involved in various cellular functions essential for development and tissue repair. Accurate measurement of bFGF/FGF2 levels is vital for understanding its impact on angiogenesis, cell proliferation, and tissue regeneration mechanisms.
This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, the kit guarantees robust performance and ease of use, making it an excellent choice for both research and clinical applications. Rely on the precision and reliability of Assay Genie's bFGF/FGF2 ELISA Kit for accurate quantification of this critical growth factor in your studies.
Product Name:
Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
SKU:
AEES00078
Target:
Human bFGF/FGF2 (Basic Fibroblast Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human bFGF/FGF2. You can calculate the concentration of Human bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
98-110
85-95
Average (%)
100
105
91
1:4
Range (%)
91-105
87-100
88-94
Average (%)
98
94
91
1:8
Range (%)
94-107
97-107
95-104
Average (%)
101
104
98
1:16
Range (%)
91-105
97-108
83-97
Average (%)
99
100
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-105
100
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
87-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
83.31
221.04
991.47
81.61
245.82
870.55
Standard deviation
4.48
10.68
48.09
4.63
11.14
36.39
C V (%)
5.38
4.83
4.85
5.67
4.53
4.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human bFGF/FGF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human bFGF/FGF2 in samples. No significant cross-reactivity or interference between Human bFGF/FGF2 and analogues was observed.
