The Human BLC (B Lymphocyte Chemoattractant) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of BLC levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.BLC, also known as B lymphocyte chemoattractant, is a key chemokine involved in the recruitment of B lymphocytes to specific tissues and sites of inflammation.
Elevations in BLC levels have been associated with autoimmune diseases, inflammation, and immune system dysregulation, making it a valuable biomarker for studying these conditions and developing potential therapies.With its high sensitivity and specificity, the Human BLC CLIA Kit offers researchers a powerful tool for accurately measuring BLC levels in biological samples, enhancing their understanding of immune responses and inflammatory processes.
Product Name:
Human BLC (B-Lymphocyte Chemoattractant) CLIA Kit
SKU:
HUES00051
Target:
Human BLC (B-Lymphocyte Chemoattractant)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BLC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BLC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BLC. You can calculate the concentration of Human BLC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
102-113
89-106
Average (%)
94
107
97
1:4
Range (%)
103-118
100-117
88-98
Average (%)
108
108
93
1:8
Range (%)
94-107
101-115
98-116
Average (%)
99
107
106
1:16
Range (%)
93-107
87-102
88-101
Average (%)
99
94
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
102-116
108
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.34
87.37
281.65
40.9
89.83
269.23
Standard deviation
4.57
7.35
24.48
4.8
6.53
21.75
C V (%)
11.92
8.41
8.69
11.74
7.27
8.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BLC in samples. No significant cross-reactivity or interference between Human BLC and analogues was observed.
