Human BLC (B-Lymphocyte Chemoattractant) ELISA Kit
The Human BLC (B Lymphocyte Chemoattractant) ELISA Kit is specifically designed for the accurate measurement of BLC levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable tool for various research applications.BLC, also known as CXCL13, plays a pivotal role in immune responses by attracting B lymphocytes to specific sites within the body.
Its dysregulation has been implicated in various autoimmune diseases, inflammatory conditions, and lymphoproliferative disorders, making it a key biomarker for studying these conditions and exploring potential therapeutic strategies.With the Human BLC ELISA Kit, researchers can confidently investigate the role of BLC in immune system functioning and disease pathogenesis, paving the way for new insights and advancements in the field of immunology.
Product Name:
Human BLC (B-Lymphocyte Chemoattractant) ELISA Kit
SKU:
HUES01340
Target:
Human BLC (B-Lymphocyte Chemoattractant)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BLC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BLC. You can calculate the concentration of Human BLC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
95-110
85-99
Average (%)
101
101
91
1:4
Range (%)
87-98
81-91
90-100
Average (%)
93
86
95
1:8
Range (%)
88-102
85-96
85-98
Average (%)
94
91
91
1:16
Range (%)
89-102
85-96
85-98
Average (%)
95
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.68
153.91
407.63
44.67
160.79
424.18
Standard deviation
3.09
7.23
13.57
2.61
6.45
14.21
C V (%)
6.48
4.7
3.33
5.84
4.01
3.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BLC in samples. No significant cross-reactivity or interference between Human BLC and analogues was observed.
