Human BMG/ beta2-MG (Beta-2-Microglobulin) ELISA Kit
The Human BMG Beta-2 Microglobulin ELISA Kit is specifically designed for precise measurement of BMG beta-2 microglobulin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for a variety of research purposes.BMG beta-2 microglobulin is a key protein involved in immune response and kidney function, serving as a biomarker for various diseases including chronic kidney disease, multiple myeloma, and autoimmune disorders.
By detecting and quantifying BMG beta-2 microglobulin levels, researchers can gain insights into disease progression, monitor treatment effectiveness, and potentially identify new therapeutic targets.Overall, the Human BMG Beta-2 Microglobulin ELISA Kit offers researchers a reliable and efficient method for studying the role of BMG beta-2 microglobulin in health and disease, advancing our understanding of its functions and implications in clinical settings.
Product Name:
Human BMG/ beta2-MG (Beta-2-Microglobulin) ELISA Kit
SKU:
HUES03084
Target:
Human BMG/ beta2-MG (Beta-2-Microglobulin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BMG/β2-MG. During the reaction, BMG/β2-MG in the sample or standard competes with a fixed amount of BMG/β2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BMG/β2-MG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BMG/β2-MG in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
92-107
99-110
Average (%)
106
98
104
1:4
Range (%)
86-97
87-102
95-109
Average (%)
91
94
102
1:8
Range (%)
88-103
95-110
98-114
Average (%)
94
100
106
1:16
Range (%)
90-103
87-103
100-116
Average (%)
95
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.6
219.6
946.4
87.0
201.8
868.1
Standard deviation
6.4
11.2
35.0
5.6
10.1
35.6
C V (%)
6.63
5.1
3.7
6.44
5.0
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of BMG/β2-MG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes BMG/β2-MG in samples. No significant cross-reactivity or interference between BMG/β2-MG and analogues was observed.
