Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit
The Human C1q and Tumor Necrosis Factor-Related Protein 3 (C1QTNF3) ELISA Kit is a reliable and accurate tool for detecting C1qTNF3 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a wide range of research applications.C1qTNF3 is a key protein involved in regulating inflammation, metabolism, and insulin sensitivity. It plays a crucial role in various physiological processes and has been implicated in diseases such as obesity, diabetes, and cardiovascular disorders.
Therefore, measuring C1qTNF3 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human C1q and Tumor Necrosis Factor-Related Protein 3 (C1QTNF3) ELISA Kit is a valuable tool for researchers looking to investigate the role of C1qTNF3 in health and disease, offering reliable and accurate results for their studies.
Product Name:
Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
