Human C1QTNF1 (C1q and Tumor Necrosis Factor Related Protein 1) CLIA Kit
The Human C1QTNF1 (C1Q and Tumor Necrosis Factor-Related Protein 1) CLIA Kit is specifically designed for the precise measurement of C1QTNF1 levels in human samples. This kit offers high sensitivity and accuracy, ensuring dependable and consistent results for a variety of research applications.C1QTNF1 is a key protein that plays a role in various biological processes, including inflammation, metabolism, and insulin sensitivity. Its dysregulation has been linked to conditions such as obesity, diabetes, and inflammatory diseases, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
With the Human C1QTNF1 CLIA Kit, researchers can effectively analyze and quantify C1QTNF1 levels in serum, plasma, and other biological samples, providing valuable insights into the role of this protein in health and disease.
Product Name:
Human C1QTNF1 (C1q and Tumor Necrosis Factor Related Protein 1) CLIA Kit
SKU:
HUES00775
Target:
Human C1QTNF1 (C1q and Tumor Necrosis Factor Related Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1QTNF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1QTNF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C1QTNF1. You can calculate the concentration of Human C1QTNF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
88-101
91-105
Average (%)
93
93
98
1:4
Range (%)
87-101
101-114
98-113
Average (%)
92
108
106
1:8
Range (%)
88-103
92-109
87-98
Average (%)
94
100
93
1:16
Range (%)
88-99
96-111
92-106
Average (%)
94
102
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
97
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.03
5.42
14.76
2.0
5.24
15.34
Standard deviation
0.2
0.58
1.11
0.18
0.41
1.09
C V (%)
9.85
10.7
7.52
9.0
7.82
7.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C1QTNF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C1QTNF1 in samples. No significant cross-reactivity or interference between Human C1QTNF1 and analogues was observed.
