The Human C4b Complement Component 4b CLIA Kit is specifically engineered for the precise measurement of C4b complement component 4b levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and dependable results, making it an invaluable tool for various research applications.C4b is a key component of the complement system that plays a critical role in the immune response by enhancing phagocytosis and inflammation.
Dysregulation of C4b levels has been associated with autoimmune disorders, inflammatory conditions, and infectious diseases, highlighting its importance as a biomarker for studying these diseases and potential therapeutic interventions.The Human C4b Complement Component 4b CLIA Kit offers researchers a reliable solution for investigating the role of C4b in health and disease, providing valuable insights into immune system function and potential diagnostic and treatment strategies.
Product Name:
Human C4b (Complement Component 4b) CLIA Kit
SKU:
HUES00504
Target:
Human C4b (Complement Component 4b)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C4b. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4b, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C4b. You can calculate the concentration of Human C4b in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
86-97
85-98
Average (%)
100
91
92
1:4
Range (%)
98-110
84-97
100-113
Average (%)
104
91
107
1:8
Range (%)
98-111
101-115
86-102
Average (%)
104
107
93
1:16
Range (%)
102-114
101-117
98-112
Average (%)
108
110
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.24
9.0
23.86
3.29
8.95
22.48
Standard deviation
0.33
0.93
1.79
0.39
0.91
1.66
C V (%)
10.19
10.33
7.5
11.85
10.17
7.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human C4b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4b in samples. No significant cross-reactivity or interference between Human C4b and analogues was observed.
